![]() ![]() Lightly oil a large bowl, place the dough in the bowl and turn to coat with oil.When the dough has pulled together, turn it out onto a lightly floured surface and knead until smooth and elastic, about 8 minutes. Add the remaining flour, 1/2 cup at a time, stirring well after each addition. Add two eggs and 1/2 cup flour beat well. Gradually add the milk and butter to the flour mixture stirring constantly.Combine milk and butter in a small saucepan heat until milk is warm and butter is softened but not melted. In a large bowl, combine 1 cup flour, sugar, salt, and yeast stir well.The Christian Easter Bread comes from the Jewish tradition but was modified over time because they did not follow kosher dietary laws.Ī recipe for Easter bread is as follows: IngredientsĢ tsp active dry or instant yeast 2/3 cup milk 2 1/2 cups all-purpose flour 1/4 cup white granulated sugar 2 tbs butter 2 large eggs 2 tbs melted butter 1 tsp salt Instructions The Jewish Challah bread is based on kosher dietary laws. The two are almost exactly the same! These recipes are similar because they come from religious backgrounds. So I decided to look up a recipe for Easter Bread to make a comparison. I kept wondering how these two breads could be so similar in taste. The challah is done when it is deeply browned. Slide the challah on its baking sheet into the oven and bake for 30 to 35 minutes, rotating the pan halfway through cooking.Be sure to get in the cracks and down the sides of the loaf. Whisk the reserved egg white with a tablespoon of water and brush it all over the challah. Place the pan somewhere warm and away from drafts and let it rise until puffed and pillowy, about an hour. Sprinkle the loaf with a little flour and drape it with a clean dishcloth. Line a baking sheet with parchment and lift the loaf on top.Pinch the pieces together again at the bottom. Braid the pieces in the pattern of over, under, and over again. Gather the ropes and squeeze them together at the very top.Roll each piece of dough into a long rope roughly 1-inch thick and 16 inches long. Place the dough in an oiled bowl, cover with plastic wrap, and place somewhere warm.The dough has finished kneading when it is soft, smooth, and holds a ball-shape. If the dough seems very sticky, add flour a teaspoon at a time until it feels tacky, but no longer like bubblegum. Turn out the dough onto a floured work surface and knead by hand for about 10 minutes.Pour the yeast mixture over the egg slurry and mix until difficult to move.Whisk these together to form a slurry, pulling in a little flour from the sides of the bowl. Make a well in the center of the flour and add in eggs, egg yolk, and oil.Whisk together 4 cups of the flour, sugar, and salt in a large bowl.Combine yeast and a pinch of sugar in small bowl with the water and stir until you see a frothy layer across the top.^ Melody of Certain Damaged Lemons (liner notes)."Blonde Redhead: Melody of Certain Damaged Lemons (20th Anniversary Edition) (Touch and Go)". ![]() "Blonde Redhead: Melody of Certain Damaged Lemons". "Blonde Redhead: Melody Of Certain Damaged Limitations". ![]() "Blonde Redhead – Melody Of Certain Damaged Lemons". "Melody of Certain Damaged Lemons – Blonde Redhead". ^ a b "Melody of Certain Damaged Lemons by Blonde Redhead Reviews and Tracks"."Rick and Morty's 'For the Damaged Coda' Scene Spawns a Savage New Meme". "Rick and Morty: 13 Best Ridiculously Weird Moments From Season 1". "A 17-year-old Blonde Redhead song is on the Shazam chart thanks to Rick & Morty". ^ a b Welsh, Caitlin (September 20, 2017).At Metacritic, which assigns a normalized rating out of 100 to reviews from mainstream publications, the album received an average score of 73 based on eight reviews. Melody of Certain Damaged Lemons was met with generally favorable reviews from critics. Critical reception Professional ratings Aggregate scores Years after the album's release, "For the Damaged Coda" gained renewed exposure after appearing in the animated TV series Rick and Morty as the recurring theme for the character Evil Morty. The final song on Melody of Certain Damaged Lemons, " For the Damaged Coda", is based on Nocturne in F minor, Op. The album was recorded at Bear Creek Studio in Woodinville, and was produced by Guy Picciotto and Ryan Hadlock. It was released on Jby Touch and Go Records. Melody of Certain Damaged Lemons is the fifth studio album by American alternative rock band Blonde Redhead. ![]()
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![]() ![]() When it comes to planning events, communication is key. Prioritize and work on the most important things firstīusy event creators have limited time, so it’s critical that they understand the most important areas to focus on and prioritize them in an event planning checklist. One defining trait of a successful event planner is that they leave nothing to chance – everything is measured and analyzed.Ģ. Party Event Planning 101Įventbrite provides a blueprint of “ 7 Things the Most Successful Event Planners Do Everyday” to help you learn how to realize success as an event party planner. Most event planners began their career path in another role within the event planning or management world and transition into a purely event planning function. Who becomes a private party event planner? These are the non-negotiables the real pros leverage to realize success in their field. The best minds in the business recognize the importance of foolproof planning systems, a step-by-step event planning checklist, and event management technology. Being an unflappable multi-tasker and negotiator are integral skills for most successful event planning professionals. To be a successful event planner, you need the ability to take on unique challenges that must be quickly overcome, often simultaneously, while thriving under pressure. How to become a successful event party planner ġ5+ Direct Deposit Agreement Examples - PDF, DOC Examplesġ7+ Advertising Contract Examples in PDF Google Docs | Pages. Ģ5+ Partnership Agreement Examples & Samples - PDF, DOC. įREE 60+ Partnership Agreement Examples & Samples in PDF DOCġ5+ Event Planning Examples [ Wedding, Birthday, College. ĩ+ Event Venue Rental Agreement Examples & Templates. ġ2+ Conveyance Agreement Examples - PDF, DOC ExamplesįREE 22+ Event Agenda Examples & Samples in PDF DOC. 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See 15+ Event Planning Examples.Ĩ+ Lawn Care Contract Examples in PDF MS Word | Pages. In order to escape issues like this, always have the contract ready. Without the proper paperwork to bind these event vendors, there is no contract that can fully explain their roles and the whole event may not work out. When you want to hire some vendors for events, you must also be careful as to how you are going to do it. The names must be written in a clear and blocked manner. This means the event vendor and the company who hired them. ![]() The complete names and details for all parties. Like in any and all agreements and contracts, the most crucial and important aspect of the document is the names and details of the parties. To create an event vendor contract, here is one thing you need to take notice of. ![]() ![]() ![]() Double the sauce if you're going to do a full 2 lb. For the default 2-lb-of beef recipe, I use just over a pound of meat instead (extra sauce). I've made this as-is twice, and about 20 times with ever-evolving tweaks. Great base recipe significant, easy tweaks needed. I bought baby carrots to add in to the beef tips, but forgot. I would also season the meat the night before. I had to stir periodically so it didn't stick to the bottom (even on simmer). Next time, I'd let it simmer for 2 or use the slow cooker so the beef becomes more tender. I brought it to a boil and then let it simmer for an hour. Then I added the gravy/broth mixture to the pot/pan. I used 1 ½ cups of beef broth instead of water. I made the gravy with the McCormick's Brown gravy packet and about ½ a Beef with Herbs gravy packet. Then I added the soy sauce and Worcestershire sauce (next time I'll use only 1/8 of a cup). Stir constantly so it doesn't burn/stick to the bottom. I tossed about 1 ½ lbs of cubed beef in the seasoned flour then browned it. Then I seasoned about ½ cup of flour with garlic powder, pepper, celery salt and a dash of Tony's Cajun seasoning. We have several different variations of pressure cooker one-pot meals with pasta.My tweaks: I sautéed the onion and about 1 ½ tsp of minced garlic in olive oil. Need more ideas? Check out What To Serve With Mac And Cheese for 15 delicious sides. Or you could eat it all on its own-it’s filling enough as is, or mix in protein and steamed vegetables for a complete one-pot meal! For special occasions, you could even serve it with lobster! ![]() I love serving this mac and cheese recipe with country style ribs or pulled pork sandwiches. Garnish with Pecorino Romano cheese and serve. Remove the lid and stir in ricotta and cheddar cheese. When cooking time ends, do a quick pressure release. This recipe cooks the noodles for 2 minutes less than the packaging suggests for the ultimate final texture.Īlso, be sure to use the quick pressure release for this recipe to avoid overcooking the macaroni! How to makeįirst of all, get an Instant Pot and gather all the ingredients.Īdd oil, pasta, salt and 4 cups of water to cover the pasta. Mushy mac and cheese is a direct result of overcooked noodles. How Do I Make Sure it Doesn’t Turn Out Mushy? Transfer to an oven-safe vessel, top with breadcrumbs, and bake at 350 degrees for about 30 minutes, or until the top is beginning to crisp. To add an easy breadcrumb topping, simply combine ½ cup breadcrumbs with 1-2 tablespoons butter in a small skillet and toast until golden brown.Īlternatively, you can bake the mac and cheese after it’s done. It makes an excellent topping for any pasta dish, including this Instant Pot Mac and Cheese. Use finely grated pecorino wherever you would use freshly grated Parmesan cheese. Pecorino Romano is an aged hard Italian cheese made from sheep’s milk that is very similar to Parmesan in terms of taste and texture. Which type of pasta to use?įor this recipe I recommend using elbow macaroni or pipe rigate like I did in the Instant Pot Pizza Pasta recipe.įeel free to choose a short pasta variety with a similar cook time. You’ll want to cook the pasta for 2 minutes less than the directions suggest. If you use gluten-free pasta, look at the box instructions and alter the cooking time accordingly. ![]() While macaroni typically contains wheat gluten, you can use gluten-free pasta to make this recipe. This recipe is vegetarian-friendly, but with three different kinds of cheese that are so important to the taste and texture of the recipe, this recipe cannot easily be made vegan. You can also check this Instant Pot Hamburger Mac and Cheese recipe. ![]() ![]() ![]() The challenge of sudoku is using the process of elimination and other strategies to identify the unique solution for the sudoku puzzle. The numbers 1 through 9 must appear exactly once in each 3x3 box. The numbers 1 through 9 must appear exactly once in each column. The numbers 1 through 9 must appear exactly once in each row. To figure out which number between 1 and 9 belongs in a particular cell, you need to adhere to the following rules: Your job is to determine the correct number to be entered in each of the empty cells. Some of the cells in the grid are prefilled with a number between 1 and 9, while many other cells are blank. This grid is subdivided into nine 3 x 3 boxes. The hard part lies in solving the puzzle!Ī sudoku puzzle consists of a 9 by 9 grid. Here's a summary of the rules of sudoku, in case you've never played before or need a refresher. Printable Sudoku Puzzles - Rules of Sudoku The solutions pages are provided as well. To print a puzzle page, click the "Print This Page" button at the top of the page. Reprinted by Permission.") The 100 Free Sudoku Printables Your readership will love it! (In your publication, simply include the following copyright statement next to the puzzle: "©. Want to play sudoku online in your browser? Visit my Free Sudoku Games page.Īre you the publisher of a newsletter, bulletin, newspaper, or magazine? Include a sudoku puzzle or word search puzzle in each issue. More Free Printables: Crosswords, Word Searches, Bible Word Searches, Special Needs Word Searches, Mormon Word Searches, Balance Quest Math Puzzles For detailed instructions, including advanced techniques, visit the Sudoku Instructions page. See below for a summary of the rules of sudoku. Or if you're a sudoku whiz, go for the harder ones. If you are new to sudoku, start with an easy puzzle. The printable sudoku puzzles pages below are listed in order of difficulty. Note: For even more sudoku, see the 100 Free LARGE Sudoku Puzzles page. ![]() There is a link to the Solutions on the puzzle page as well. Playing sudoku daily can boost your concentration, ease depression and even prevent Alzheimer's disease.On this page are 100 free sudoku puzzles I made that you can print out right now. Can you imagine that there are 5,472,730,538 possible sudoku puzzles? The Times in London helped spread Sudoku to Western culture by publishing its first daily sudoku in 2004. Therefore, in the sudoku game, we have to fill in numbers without repetition. What is Sudoku? 'Su' in Japanese means numbers while 'Doku' means single. The best part of the game: This free online sudoku game delivers a new puzzle each and every day! Interesting Facts about Sudoku ![]() The goal of the game is to fill every square on the grid with a number from 1-9, where the numbers can only appear once in every row, column and 3x3 box. You can place notes, find sudoku tips, manage the timer and save the sudoku game by clicking the menu bar in the top-right corner. ![]() Worried about damaging your sudoku puzzle with eraser marks? When you play our free online sudoku game, there's no need! You can choose easy sudoku, medium sudoku, or even sudoku for experts if you are confident. ![]() ![]() ![]() They're both bad but atleast Soul Calibur 5 didn't aim to hit my wallet as hard. Soul Calibur 5 was badly executed but Soul Calibur 6 was a selling out on top of being bad. ![]() Playing a vanilla character was a distinct disadvantage and it should not be like that! I know that it's a fighting game and the story isn't it's strength but it shouldn't be this bad that it is distracting.Ĭustom characters were the way to go in SC6 multiplayer because you could outfit your character in such grotesque proportions that you obscured your hit boxes and gave a smaller telegraphed window to have your attacks blocked. And the entire plot line is cookie cutter predictable. The voice acting is cheesy and overacted. ![]() The dialogue is horrendous in how basic it is. I played the game solo for the most part or when I did play with other people it was with my steam friends. Only legit way you're playing 2 or 4 is if you purchase a hard copy of 4(digital ver and all DLC taken down) and buy 2:HD on the Xbox or PS3 stores. Originally posted by Baby□:You gotta on some hard stuff to think SC6 is anywhere near as bad as 5. But do you know of anyone who ever actually was caught for using emulators for games they didn't own? I feel like its a J-walking crime, its against the law but nobody ever enforces it. Not sure exactly where they are but I did buy them. They could be back at my parents place or my brother could have swiped them when he shipped off to college. I did buy the older titles but that was decades ago now. I can only imagine that an SC 7 would double down on cash grabbing and put out an even less polished product. I bought it with the hope that doing so might keep the series alive after the flop that was SC 5 but after playing SC 6. Yeah, not a fan of cash grab games that cut corners. Unfortunately, there's no way to legally play older titles on PC unless you own the console discs. It is the worst installation in the series for me and I hate admitting that because Soul Calibur 5 exists.Wow. DLC characters on day 1, cosmetic content that was copy pasted from previous titles also obnoxiously behind a pay wall and the story mode dialogue was the pure essence of cringe. Unlike Arcade mode each bout of Story Mode consists of fighting 3 or four enemies in a single round except when fighting a boss.Originally posted by KalkiKrosah:I really hated how Soul Calibur 6 was brought to the market. In Story Mode characters generally either seek to acquire Soul Calibur (the benevolent sword) or destory Soul Edge (the malevolent sword) for those with positive alignment, and the opposite for characters with negative. Each character is assigned either positive or negative alignment, which only affects gameplay slightly for the skills which either increase or decrease depending on like or dissimilar alignment of the characters fightering. The Story Mode starts with a text introduction, there is a mini-boss half way through which is always one of 4 characters depending on the character the player has selected, and finally the final boss which is usually Algol. The guest characters are from the Star Wars Universe: Apprentice, Darth Vader and Yoda (Vader must be purchased as dlc for XBOX, and Yoda for Playstation).Įach character's story is told in Story mode. The game is a Platinum Hit for X360 and Greatest Hit for Playstation. Finally, local or online multiplayer on XBoxLive or the PSN network with or without clothing effects and one character for each player. There are 4 single player modes, Story, Tower of Lost Souls, Arcade and Versus CPU. Character clothing and physical features may be customized where the articles of clothing add different stats to the character which are then used to equip skills. Elements include a "Soul Gauge" which depletes each time a player blocks an attack and increases each time he or she strikes the opponent, which if depleted leaves the player vulnerable to a "Critial Strike" which ends the round. It was originally released on PlayStation 3 and Xbox 360. Soulcalibur IV is a 3-D fighting game featuring armed combat and eight directions of movement. ![]() ![]() The Sting-Ray Classic was the most expensive model at $395.00. The Sting Ray was available in three different model: It had a reputation as being light weight and easy riding. The Schwinn Sting Ray was introduced in 1964 and became the company's best selling bike. It has features like fast starts and short radius turn benefits. Lightweight Schwinn Sting-ray was featured with adjustable handles and had bucket saddles that can fit 5 to 6-foot riders. ![]() Front and rear suspension were provided by articulating forks in the front, while a spring-loaded sissy bar in the back provided a "floating seat." Up front, a drum-style front brake was installed, while the rear was equipped with a mechanical disc brake. These bikes included features that were cutting-edge not just for the Sting-Ray line, but for bicycles in general. The unusual stick shift placed on the top tube, directly between the saddle and the handlebars, was what really set the bike apart. In reality, in 1968, the name Krate was taken from a prominent California dragster. Dragsters were to vehicles what the Krate was to the Sting-Ray. Schwinn released the Schwinn Krate, the most recognizable version of the Sting-Ray series to date, in 1968. The bike was more fun with new glow bucket saddles, chrome rods, back tires, and trigger control. The hand brakes were available only on 3-speed Schwinn sting-ray frame models. The sting-ray bike features high and long handles, a short frame with the bucket-shaped saddle. These Vintage Schwinn Sting-ray Bikes were known as "bike with sports car look" which transformed cycling as soon as they were introduced in the market. Here is how Schwinn sting-ray's models changed cycling. The original Schwinn sting-ray reproduction began between 1963 to 1981. Let's take a look at Schwinn sting-ray bikes history. In the 1960s, the Sting-Ray became synonymous with suburban youth. The bike's name was inspired by the (at the time) new Corvette, further cementing the link between the bicycle and the 1960s' exaggerated vehicles. The huge back wheel of children's bikes had a coaster brake, and the wide-stance handlebars were ideal for popping massive wheelies. The Sting-Ray became Schwinn's best-selling bike in a short period of time. The Sting-Ray was proposed by Al Fritz, Schwinn's head of research and development, as a means to recreate the aesthetic California youth were seeking for. Schwinn didn't take long to design a comparable look. ![]() 20-inch wheels, enlarged seats, rear "sissy bars," and ape hanger handlebars were also standard on the bikes. The idea is to print them up, so you have a handy reference.The Krate was the trendiest and most desired of the Sting-Rays, a bike that brings a grin to the faces of those who previously had it.Īfter learning that teenagers in California were modifying their bikes to appear like motorbikes, Schwinn launched the first Sting-Ray in 1963. The following pages were created by Brian Crawford with the intent of sharing information with all bicycle enthusiasts! He holds the copyright on them, starting in the year (©)2001 to the present day. Schwinn Serial Numbers, The Complete List! Bunch "O" Bikes, Where To Find Schwinn Serial Numbersįind your numbers? Now go here to find what those numbers mean!! ![]() ![]() ![]() Hit the Generate button and your logo/image is created and ready to download. Enter your text, select a font, choose a font size, and pick your favorite colors. Use our free Georgia font generator tool below to create your own custom design logo or image. Total Files Included: 4 (regular, bold, italic, bold italic).Georgia font is available and ready to go free of charge via the download button below. Available in TTF and OTF formats, with well-designed kerning and ligatures, Georgia has become a staple for text-heavy documents and is often paired with sans-serif display fonts for contrast. This versatile typeface excels in various applications, from web and mobile interfaces to print media such as magazines and academic journals. Characterized by its robust, legible structure and slightly rounded serifs, Georgia strikes a balance between formal elegance and readability. The font-family includes a variety of weights and styles, such as regular, italic, and bold, offering a versatile range of options for typographic pairing. Furthermore, it makes the downloaded Georgia font compatible with your browser.Georgia is a serif typeface designed by Matthew Carter in 1993 and commissioned by Microsoft. It let you create different and eye-catchy Georgia font Logos and posters. There are different platforms where you can easily consider using this font including Texts, Books, Trading platforms, Headings, Websites, etc. This typeface is not an open-source font, thus there are two versions available: one is paid for, and the other is free. It was designed by a British designer Matthew Carter who is already known for designing many effective and appealing typefaces. The Georgia font was first introduced with the release of Windows 95, and it quickly became a widely used font for digital content, including web pages and e-books. Once you have the license, the usage will be unlimited. In order to use the font on the commercial and Trading level, you need to go for the license first. The font is free to use only for your personal projects. Since it releases, various weights and versions have been produced with time. The designer took inspiration from the 19th-century notable design Scotch Roman. It emerged in 1993 by a British designer Matthew Carter. It is a serif typeface that is known for making small-size texts readable. GL Kupiura GL Gordeziani GL Chonchkhi GF Alaverdi Eurostile LT. GeoPerf Regular: GeoLab fonts GL Venuri GL Mkafio GL Lortkipan. Georgia Font Viewĭownload FAQs (Frequently Asked Questions) Fonts: Packs: Bakuri Italic Bakuri Regular DM Medea Reg. Is there any font similar to Georgia in Google Fonts Ans: The most similar Google font to Georgia is. Is Georgia Font safe to download and use on My PC and MAC Ans: Yes, Georgia is 100 safe to download and secure to use on any device and operating system. TTF and OTF are the 2 common formats in which you can download this font. Ans: Georgia is a serif typeface font that is used in The New York Times magazine. It comprises different font weights concluding Bold, Italic, Regular, etc. It makes the small size text understandable.įurthermore, it is highly used for books, Headings, text, Designs, websites, and many other places. For printing purposes, where the text is in small size, Georgia font is perfect for such a platform because of being a highly-legible font. Im weitesten Sinne handelt es sich um Serifen Schriften. Font Meme is a fonts & typography resource. Es gibt nun Schriften welche auf Google Fonts gelistet sind, welche der Georgia sehr ähnlich sind. Ich persönlich liebe die Schriftart Georgia. Use our free Georgia font generator tool below to create your own custom design. Format: TTF (True Type Font) Total Files Included: 4 (regular, bold, italic, bold italic) Download. Font Name: Georgia License: Free for Personal Use. Like every other serif font, it also has a long list of platforms where it is used since it releases. Dieser Beitrag zeigt euch 5 Schriftarten, welche wie die Schriftart Georgia sind. Georgia font is available and ready to go free of charge via the download button below. This font duo also support multilingual, number and symbol, end swash and many ligatures. Contain two fonts, the delicate modern serif and a free hand writing script. Besides all this, it is entirely free to use without any font requirement. Georgia Ballpark is perfect combination between modern serif with signature script font. The tool is also used to eliminate the compatibility issue with the font and browser. Furthermore, it comprises an online to with the name Georgia font Generator that allows you to produce different font Posters and Logos. It has different similar fonts that can be contemplated instead of Georgia font including Baskerville font and Crimson Semibold font. It has different traditional features that make this typeface elegant and flawless. ![]() ![]() ![]() EfimovaI, E.N.Guseva, N.N.Kostina, L.A.Saveleva, I.I.Bobrova, “Modeling of students' competency development in the higher education distant learning system”, Proceedings of the 2016 Conference on Information Technologies in Science, Management, Social Sphere and Medicine (ITSMSSM 2016), p. Kolesnikova, “Simulation of Production Cycle of Wire Production”, Vestnik Samara State Aerospace University, T.14, No. Sabitov, G.S Smirnov, “Imitation modeling of the relay delivery system”, Bulletin of the Scientific Center for Life Safety, No. Haverkort, “An AnyLogic simulation model for power and performance analysis of data centres”, Proceedings of the 12th European Workshop on Computer Performance Engineering, EPEW 2015. Yıldız Technical University, Industrial Engineering Department, Istanbul, 2014 (in English).ījörn F. Gizem Gunes, Ali Fuat Guneri, “Agent-based simulationand an example in Anylogic”. North, “Tutorial on Agent-Based Modelling and Simulation”, The University of Chicago, 2010. Borshchev, “The Big Book of Simulation Modeling: Multimethod Modeling withAnyLogic” 6, 2013 (in English).Ĭ.M. The virtual model of the technological line allowed testing the future technological line for adequacy, process ability and reliability.Īs a result of studying the discipline "CAD of packaging production" and AnyLogic software, students develop holistic understanding of the automated process and acquire the skills of modeling, analysis and optimization of the technological line.Ī. With the help of the software of The AnyLogic Company, students create an acting part of the model, simulate processing at workshops and the work of a warehouse.Īs a result, in the developed simulation model, the authors researched the load of the production elements, determined problematic structural elements and calculated the coefficient of the technological line readiness. ![]() A formal description of the packing line has been made for a more detailed understanding of the process and the adequate construction of the model. The parameters for constructing the simulation model are selected. The criteria that influence the technological process of bulk products packing are determined. Authors highlighted the stages of modelling a flexible automated packaging system. The methodology for developing a flexible automated line simulation model, for example, for the production of macaroni products, is described in this work. The study of technological processes based on simulation modeling allows the students to identify interconnections, to highlight technical and economic indicators, to evaluate the impact on profitability, to determine the directions of development and ways to improve the technical and technological facilities. The purpose of modeling the technological processes of packaging is to increase their productivity by determining and justifying the optimal parameters. As a result of studying the software product, the students will be able to master the techniques of automated calculation and design of packaging components and implement projects using modern CAD and CAM systems. The paper presents software products for automated calculation and modeling of the technological process of packaging. Applied Mechanics” in the course “CAD of packaging production” using the specialized software of the AnyLogic Company. The authors have considered possibilities of organizing the training of students majoring in engineering specialty “131. ![]() ![]() ![]() In this post we’ll look at the best way to acquire and analyse the humble Western blot.Ī quick thanks to Rosalie Richards (of the Sée Lab) for supplying the lovely western blot used as an example in this post. Instead of using fluorescent labelled antibodies ( although this can be done), most WBs use ChemiLuminescence to detect the amount of protein present. Proteins are separated based on their size then labelled and identified using antibodies. Your standards should be linear.The Western blot is a staple of many Research Labs.Use the trendline formula to solve for your unknown proteins.Save your Results window so that you can transfer the measurements to excel to generate a standard curve You should fix this as soon as you click on the wrong part of the histogram to keep your numbering correct.ġ3. If you click on the wrong part of the histogram, you can find the bad measurement in the Results window, highlight it, go to Edit→cut and remove the bad entry.Be aware that ImageJ isn't "smart" - The order in which you click corresponds to the numbers in the Results window numbering does NOT correspond to the lanes you defined!.The measurement of the areas will be bumped to a "Results" window.Continue selecting the area outlines of the remaining lanes On the ImageJ interface, select the "magic wand" button and then click on the line defining the area of the curve of the first standard, and the areas of the curves in your protein analysis lanes Therefore, three curves have been established.ġ2. ![]() In this example, we know that the protein consists of 3 subunits, and they represent the majority of the protein sample. Draw a line at the bottom of the peak that represents the first standard to define the area of the curve - Keep drawing all the single lines to define the curves in your standard lanes, and draw multiple lines in your lanes with your protein of interest. On the ImageJ interface, select the "line" button The peaks (or valleys if your image is inverted like the one in this example) in each grid correspond to the intensity of the bands in the lane The standards lanes should only show one peak, while the lanes with protein you want to quantify will probably show multiple bands, as in the image below - Now is a good time to save! If the plot window is active, when you go to File→Save through the ImageJ interface, it will be trying to save your histograms if the image window is active, the image will saved. Once all lanes are defined, go to Analyze→Gels→Plot lanes (or use "Ctrl+3") to generate histograms of each lane If you are some kind of wizard who knows how to fix these mistake boxes without having to start over again, you should let me know!ġ0. You will get really good at marking the lanes, I promise. ![]() My best advice if you find yourself in that predicament is to close the file, reopen it and start again. I think at this stage it's easiest to use key command "Ctrl+2" to continue numbering the subsequent lanes (less of a chance to mess up!) - I do not know how to correct the inevitable mistake boxes that you are going to make by accident and that cannot be undone.I'm sorry! Deleting them will cause useless white space where the rectangle was previously. Repeat steps 7-8 until all lanes have been selected and numbered Go to Analyze→Gels→Select next lane - Can also use key command "Ctrl+2" - A tiny “2” will appear in the laneĩ. DO NOT DRAW A NEW RECTANGLE! You must drag the same rectangle you just made - The point here is to compare the band in each subsequent lane using the exact same size/white space/noise as the originally defined area in Lane 1Ĩ. Make sure your cursor shows as an arrow, grab the rectangle you just made, and drag it to the next lane Go to Analyze→Gels→Select first lane - Can also use key command "Ctrl+1" - A tiny “1” will appear in the laneħ. Select the rectangle tool, and draw a box around the lane, making sure to include some of the empty gel between lanes and white space outside of the bandĦ. Find the lane with the lowest concentration of BSAĥ. Image→Adjust→Brightness/contrast - I recommend saving the image with an updated name at this point so that you have it to go back toĤ. Does your image look too dark or too light?.Make sure you save your gel images as the same type of image (either. jpg (in case the tif file can’t be opened-an issue I am experiencing at the other lab). After running and destaining the gel, take a picture and save it as a.For 5GB1, BSA works great as a protein standard, and a range of 0.025 μg/μL to 5.0 μg/μL works well as a range for the standard curve To determine protein concentration you will need to have a standard curve to compare your samples to.Determining the concentration of protein in SDS-PAGE gel bands using ImageJ ![]() ![]() Before you go don’t forget to bookmark the site so you an always get working Krunker Hacks and Cheats. 14) Enjoy! You are all done and ready to go.13) Go to the menu and click the cheats icon on the bottom left to enable the cheats.12) Head over to Krunker.IO or refresh the game and you will now have working krunker.io aimbot and hacks within the game.Then scroll down on the page to the experimental section and change the inject mode to instant. Copy and paste this script into a new AutoHotkey File and run it. 11) Click on the settings tab, on the general section change the config mode to advanced. ![]() You should see the script be active on the dashboard.
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